Since e-Learning is one of the Critical Agenda Projects (CAPs) and a National Key Result Area (NKRA) of the Malaysian Ministry of Higher Education (MOHE), a comprehensive study on the implementation of e-Learning in institutions of higher learning (IHLs) was recently conducted and funded by MOHE. This keynote will highlight key findings of this nation-wide study aimed at exploring the status, trends and challenges of e-Learning implementation in Malaysian IHLs. A total of 26 e-Learning administrators, 1,635 lecturers, and 6,301 students from selected Malaysian IHLs were involved as respondents. Data were obtained from the Malaysian IHL e-Learning Questionnaire or MIeLQ (IT Manager) which contains 74 items, the MIeLQ (Instructor) which contains 35 items, and the MIeLQ (Students) which contains 20 items. The main aspects investigated include: (i) policy, ii) governance, iii) Learning Management System (LMS), iv) training, (v) e-Content development, and (vi) integration of e-Learning in teaching and learning. Findings will be presented in terms of a) status, b) trends, and c) challenges of e-Learning implementation in Malaysian IHLs.